Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping

Parallel implementation of finite volume based method for isoelectric focusing

A message passing interface (MPI) based parallel simulation algorithm is developed to simulate protein behavior in non-linear isoelectric focusing (IEF). The mathematical model of IEF is formulated based on mass conservation, charge conservation, ionic dissociation-association relations of amphoteric molecules and the electroneutrality condition. First, the concept of parallelism is described f...

Apolipoprotein E polymorphism determined by restriction enzyme analysis of DNA amplified by polymerase chain reaction: convenient alternative to phenotyping by isoelectric focusing.

Three common alleles determine six apolipoprotein E (apo E) phenotypes that are associated with variations in serum cholesterol in the population. This genetic variation results from single nucleotide alterations at two DNA loci encoding the amino acid residues 112 and 158 of apo E. We compared results of apo E phenotyping carried out by isoelectric focusing with those of apo E genotyping accom...

Isoelectric Focusing of Serum Apolipoprotein C-III as a Sensitive Screening Method for the Detection of O-glycosylation Disturbances.

Apolipoprotein C-III (ApoC-III) is a glycoprotein carrying the most common O-linked glycan structure and is abundantly present in serum, what renders it a suitable marker for analysis of O-glycosylation abnormalities. Isoelectric focusing followed by a Western blot of ApoC-III, using PhastSystem™ Electrophoresis System (GE Healthcare), was introduced as a rather simple and rapid method for scre...

Isoelectric focusing.

Immunofixation following electrophoresis or isoelectric focusing for identification and phenotyping of proteins.

Immunofixation following electrophoresis or isoelectric focusing has proven to be a powerful tool for the phenotyping of plasma proteins, the identification of proteins such as M-components and the study of conversion or breakdown products of proteins such as C3. One of the major advantages is the ability to compare, side by side, the mobilities of relatively low-concentration proteins. The tec...